Overview

The expressionfactory provides the complete solution to the problems associated with protein expression.

• Large scale production of any one or many selected proteins (from > 10,000) to high purity.

With a unique combination of biology, software and instrumentation, the system fully automates and integrates the processes involved. Vector construction, protein expression and purification are performed within a single platform. Expression of a particular protein can be difficult to achieve. The processes involved are complex, and specific proteins may require individual strategies for optimal expression. The unique features of the expressionfactory overcome these difficulties and limitations, and improve protein expression.

The expressionfactory adopts a matrix approach to protein expression and enables the user to investigate different combinations of vector construction, hosts used to express the protein, growth conditions and purification strategies, in a single run. The data generated is organised and controlled by the information management system (IMSTM) underpinning the expressionfactory, which removes the sequence, sample and protein-tracking problems usually associated with highly parallel expression.

 

The multi-vector strategy enabled by the expressionfactory allows the user to define the expression method which best facilitates production and purification of protein fit for purpose. In addition, the system provides the facility to engineer proteins to express domains of the molecule when the full length protein is insoluble or will not express. These features, in combination with the platform's advanced automation and software facilities, result in the systematic and parallel production of many hundreds of proteins in minimal time.

 

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Location:

CBNI, UCD